IgD Monoclonal Antibody (IA6-2), FITC, eBioscience

货号：11-9868-42

规格：100 tests

价格：2518

产品类型：流式抗体

品牌：eBioscience

抗原：IgD

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：IA6-2

荧光染料：FITC

类型:	人B细胞流抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience
浓度：	5μL/test	用法：	5μL(0.125μg)/test(Flow)

产品详细信息Description:This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD). This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.Applications Reported: This IA6-2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This IA6-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

IgD Antibody (11-9868-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human IgD FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.JCI insightB cells from African American lupus patients exhibit an activated phenotype."11-9868 was used in Flow cytometry/Cell sorting to investigate immunological differences between African American and European American patients with systemic lupus erythematosus, showing that B cells from African American patients exhibit an activated phenotype."2.Frontiers in immunologyCD19+CD24hiCD38hiB Cells Are Expanded in Juvenile Dermatomyositis and Exhibit a Pro-Inflammatory Phenotype After Activation Through Toll-Like Receptor 7 and Interferon-α.

技术参数

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