CD8a Monoclonal Antibody (RPA-T8), PE, eBioscience

货号：12-0088-80

规格：25 µg

价格：1416

产品类型：流式抗体

品牌：eBioscience

抗原：CD8

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：RPA-T8

荧光染料：PE

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	0.2 mg/mL	用法：	0.25 µg/test (Flow）

产品详细信息Description:The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: The RPA-T8 antibody been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD8a Antibody (12-0088-80) in FlowStaining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD8a PE and CD4 PerCP-Cy5.5. As expected based on known relative expression patterns, CD4 clone OKT4 (OKT-4) stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

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参考文献：

1.The Journal of biological chemistryCD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers."12-0088 was used in Immunofluorescence to investigate raft localisation into CD8αβ heterodimers versus CD8αα homodimers in human cells."2.Stem cells internationalUmbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria."Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry"3.Central-European journal of immunologyCD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients."Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry"

技术参数

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