CD57 Monoclonal Antibody (TB01 (TBO1)), PE-Cyanine7, eBioscience

货号：25-0577-42

规格：100 Tests

价格：3163

产品类型：流式抗体

品牌：eBioscience

抗原：CD57

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TB01 (TBO1)

荧光染料：PE-Cy7

抗体类型:	流式抗体	同型对照：
浓度：	5 µL/Test	用法：	5 µL (1 µg)/test (Flow）

产品详细信息Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.Applications Reported: This TB01 (TBO1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD57 Antibody (25-0577-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0036-42) and 1.0 µg of Mouse IgM Isotype Control APC (Product # 17-4752-80) (left) or 1.0 µg of Anti-Human CD57 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Cell reportsMass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells."Published figure using CD57 monoclonal antibody (Product # 25-0577-42) in Flow Cytometry"2.AutophagyA novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells."Published figure using CD57 monoclonal antibody (Product # 25-0577-42) in Flow Cytometry"

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