IgM Monoclonal Antibody (II/41), APC,

货号：17-5790-82

规格：100 µg

价格：2892

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：APC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), APC
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: The II/41 antibody has been reported for use inflow cytometric analysis.Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (17-5790-82) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse IgM APC (right). Cells in the small scatter population were used for analysis.

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参考文献

1. The Journal of experimental medicineElucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region.2. Cell reportsModest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal.3. Molecular medicine reportsAnti‑IL‑39 (IL‑23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus‑like mice.4. International journal of molecular sciencesModulation of Innate Immunity by lignin-Carbohydrate, a Novel TLR4 Ligand, Results in Augmentation of Mucosal IgA and Systemic IgG Production.5. Nature communicationsExtrafollicular CD4+T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

技术参数

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