CD80 (B7-1) Monoclonal Antibody (16-10A1), Biotin, eBioscience

货号：13-0801-82,13-0801-85

规格：100 µg,500 µg

价格：1577,2580

产品类型：流式抗体

品牌：eBioscience

抗原：CD80

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：16-10A1

荧光染料：Biotin

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD80 (B7-1) Antibody (13-0801-82) in FlowStaining of 3-day LPS-stimulated C57BL/6 splenocytes with 0.06 µg of Armenian Hamster IgG Isotype Control Biotin (Product # 13-4888-81) (open histogram) or 0.06 µg of Anti-Mouse CD80 (B7-1) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

1. Journal of immunology (Baltimore, Md. : 1950)Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell differentiation and induces autoimmunity."13-0801 was used in Flow cytometry/Cell sorting to investigate the molecules that regulate dendritic cells to protect against autoimmunity, showing that loss of Shp1 promotes Th1 cell differentiation and induces autoimmunity."AuthorsKaneko T,Saito Y,Kotani T,Okazawa H,Iwamura H,Sato-Hashimoto M,Kanazawa Y,Takahashi S,Hiromura K,Kusakari S,Kaneko Y,Murata Y,Ohnishi H,Nojima Y,Takagishi K,Matozaki TYear2012SpeciesMouse 2. Journal of immunology (Baltimore, Md. : 1950)Exposure to nicotine adversely affects the dendritic cell system and compromises host response to vaccination."13-0801 was used in Flow cytometry/Cell sorting to investigate the causal relationship between nicotine-induced dendritic cell alterations and host response to vaccines."AuthorsNouri-Shirazi M,Guinet EYear2012SpeciesMouse 3. Infection and immunityToxoplasma gondii induces B7-2 expression through activation of JNK signal transduction."13-0801 was used in Flow cytometry/Cell sorting to determine molecular mechanisms governing the induction in expression of costimulatory ligands during Toxoplasma gondii infection."AuthorsMorgado P,Ong YC,Boothroyd JC,Lodoen MBYear2011 4. BloodAlternatively activated macrophages inhibit T-cell proliferation by Stat6-dependent expression of PD-L2."13-0801 was used in Flow cytometry/Cell sorting to study how IL-4 induced the expression of PD-L2 on CAM, and LPS increased the expression of PD-L1 on alternatively activated macrophages."AuthorsHuber S,Hoffmann R,Muskens F,Voehringer DYear2010SpeciesMouse 5. The Journal of clinical investigationSelective ablation of basophils in mice reveals their nonredundant role in acquired immunity against ticks."13-0801 was used in Flow cytometry/Cell sorting to investigate the cellular and molecular mechanisms underlying acquired resistance to Haemaphysalis longicornis ticks in mice."AuthorsWada T,Ishiwata K,Koseki H,Ishikura T,Ugajin T,Ohnuma N,Obata K,Ishikawa R,Yoshikawa S,Mukai K,Kawano Y,Minegishi Y,Yokozeki H,Watanabe N,Karasuyama H

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