Mouse anti Human CD2:Low Endotoxin
货号：MCA1194EL
规格：0.5 mg
价格：5100
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD2
物种：人
宿主：小鼠
抗体亚型：IgG2b
克隆号：LT2
荧光染料：Low Endotoxin
| 产品介绍 | | |
| Mouse anti Human CD2 antibody, clone LT2recognizes human CD2, a single chain transmembrane glycoprotein of 50 kDa, expressed by T cells, NK cells and thymocytes.CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells.Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rhesus Monkey | √ |
| Chimpanzee | √ |
| Cynomolgus monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None presen |
Immunogen | Normal human peripheral blood granulocytes. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20oC only.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/50 | 1/100 |
Functional Assays | √ | | |
Immunohistology - Frozen1 | √ | 1/200 | 1/400 |
Immunohistology - Paraffin | × | | |
Immunoprecipitation | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
- Histology Positive Control Tissue
- Human Tonsil
|
| 同型 |
Description | Product Code | Applications | Pack Size |
Mouse IgG2b Negative Control | MCA691 | F | 100 Test |
| 文献 |
| 1. Moingeon, P.et al.(1989) The structural biology of CD2.Immunol Rev. 111: 111-44.2. Beyers, A.D.et al.(1989) Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen.Immunol Rev. 111: 59-77.3. Lin, C.W.et al.(2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.Blood. 106 (10): 3567-74.4. Kap, Y.S.et al.(2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.J Histochem Cytochem. 57: 1159-67. |
技术参数 产品应用 Flow;IP;IHC(F);FN
产品类型 Monoclonal Antibody
推荐用于 Flow;IP;IHC(F);FN
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