Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody
货号：STAR73
规格：0.5 ml
价格：4956
产品类型：流式抗体
品牌：Bio-Rad
物种：大鼠
宿主：山羊
抗体亚型：IgG
荧光染料：PE
| 产品介绍 | | |
| Phycoerythrin conjugated Goat F(ab')2anti Rat IgG antibodyrecognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues.We recommend diluting Phycoerythrin conjugated Goat F(ab')2anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity. |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | F(ab')2fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 0.5 ml distilled water |
| Antiserum Preparation | Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Whole rat IgG. |
| Approx. Protein Concentrations | IgG concentration 0.05mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/5 | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
| 文献 |
| 1.Bellingan. G.et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.J. Exp. Med. 196:1515-1521.2.Broderick, C.A.et al.(2005) Local Administration of an Adeno-associated Viral Vector Expressing IL-10 Reduces Monocyte Infiltration and Subsequent Photoreceptor Damage during Experimental Autoimmune UveitisMol Ther. 12: 369-73.3.Ko, Y.C.et al.(2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell–endothelium interactionsJ Anat. 214: 183-95.4.Curran, C.S.et al.(2006) Lactoferrin activates macrophages via TLR4-dependent and -independent signaling pathwaysCell Immunol. 242: 23-30. |
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产品类型 多克隆抗体
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