| 产品介绍 | | |
| Recombinant human FGF8is anE. coli-derived protein corresponding to amino acids 23-215 ofhuman fibroblast growth factor 8. FGF8 is a secreted peptide that promotes cell growth in an autocrine manner.The peptide sequence contains an initiator methionine at the N-terminus and is homologous to the murine, canine and bovine sequences.PHP130 has an ED50of <0.5ng/ml as determined by dose-dependent thymidine uptake in BaF3 cells expressing FGF receptors. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Reconstitution | Reconstitute with 0.25 ml distilled water.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% Bovine Serum Albumin is recommended. |
| Preservative Stabilisers | None present |
| Carrier Free | Yes |
| Purity | >95% by SDS PAGE and HPLC analysis |
| Protein Molecular Weight | 22.4 kD (193 amino acid sequence) |
| Approx. Protein Concentrations | Total protein concentration 0.1 mg/ml after reconstitution |
| Regulatory | For research purposes only |
| Guarantee | 3 months from date of reconstitution |
| 存储条件 | | |
| Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | 0.1ng/ml | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
QQ:1749072012