| 产品介绍 | | |
| Recombinant human CD253is a 19.6 kDa protein comprised of 168 amino acids which correspond to the TNF-homologous region of the extracellular domain of human TRAIL/ApoL2.TNF-related apoptosis-inducing ligand (TRAIL) is expressed in many tissues including spleen, lung and prostate (Wileyet al, 1995) and is capable of inducing apoptosis in a tumor specific manner (Walczaket al, 1997). Apoptosis is initiated by the binding of the two death receptors DR4 and DR5 which initiate cell death signaling via the caspase cascade. There are however two decoy receptors DcR1 and DcR2 which competitively bind to CD253 but do not initiate apoptosis (Allen and El-Deiry, 2012).A potential use for TRAIL as an anti-tumor therapeutic agent has been identified, demonstrating tumoricidal activity in approximately two thirds of tumor cell lines tested (Griffithet al, 1999).Recombinant human CD253 is the immunogen used to produce Rabbit anti human CD253 (AHP1215). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Reconstitution | Reconstitute with 0.1 ml distilled water. For extended storage, the addition of 0.1% BSA is recommended.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Preservative Stabilisers | None present |
| Carrier Free | Yes |
| Purity | >98% by SDS PAGE and HPLC analysis |
| Protein Molecular Weight | 19.6kD (169 amino acid sequence) |
| Approx. Protein Concentrations | 0.5 mg/ml after reconstitution. |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Prior to reconstitution store at -20℃. Following reconstitution store at -20℃.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | 0.2 | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
QQ:1749072012