Mouse anti Sheep MHC Class I monoclonal antibody, clone VPM19
货号：MCA897GA
规格：0.1 mg
价格：2151
产品类型：流式抗体
品牌：Bio-Rad
抗原：MHC Class I monoclonal
物种：绵羊
宿主：小鼠
抗体亚型：IgG1
克隆号：VPM19
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Sheep MHC Class I antibody, clone VPM19recognizes the ovine homologue of the human MHC Class I, a monomorphic determinant expressed on the heavy chain of sheep MHC Class I, (OLA Class I).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In sheep, this is often referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC Class I functions in the recognition and presentation of foreign antigens to T-cells.Ovine MHC Class I is a membrane glycoprotein with a molecular weight of approximately 44kDa, expressed on the cell surface of all peripheral blood leucocytes.Clone VPM19 has been in used in a number of domestic animal disease states, in particular Maedi Visna virus infection, a disease of significant importance in commercial sheep flocks (Leeet al.1996,Ryanet. al.2000andWuet. al.2008). Mouse anti Sheep MHC Class I antibody, clone VPM19 recognizes MHC class I in other species and has been used in a study of feline herpes virus infection (Montagnaroet. al.2009). |
| 产品详情 | | |
| Target Species | Sheep |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Sheep T cells. |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS0 myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide (NaN3) |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/25 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | F | 0.5mg |
| 文献 |
| 1.Montagnaro, S.et al.(2009) Feline herpesvirus-1 down-regulates MHC class I expression in an homologous cell system.J Cell Biochem. 106: 179-85.2.Wu, C.et al.(2008) Mapping and characterization of visna/maedi virus cytotoxic T-lymphocyte epitopes.J Gen Virol. 89 (Pt 10): 2586-96.3.Chan, S.S.et al.(2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.Immunology. 107: 366-72.4.Ryan, S.et al.(2000) Infection of dendritic cells by the Maedi-Visna lentivirus.J Virol. 74 (21): 10096-103. |
技术参数 产品应用 IHC(F);Flow;IP;WB*
推荐用于 单克隆抗体
组成成分 IHC(F);Flow;IP;WB*
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