| 产品介绍 | | |
| Mouse anti Chicken CSF1R, clone ROS-AV170, recognizes the chicken homologue of human CSF1R, also known as CD115, CSF-1R, M-CSF receptor and c-fms. CSF1R is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceauet al.2010).CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cells (Garceauet al.2010).Mouse anti Chicken CSF1R, clone ROS-AV170 is a monoclonal antibody that has been generated to recognize chicken CSF1R . Clone ROS-AV170 has been shown to recognize the CSF1R antigen in some, but not all closely related avian species.Mouse anti Chicken CSF1R, clone ROS-AV170 is an excellent marker for chicken macrophages. |
| 产品详情 | | |
| Target Species | Chicken |
| Product Form | Purified IgG conjugated to Alexa Fluor 488 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Purified ChCSF1R-Fc fusion protein |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Fusion Partners | Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Garcia-Morales, C.et al.(2014) Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells.Dev Comp Immunol 42:278-85.2.Garceau, V.et al.(2015) The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor.BMC Biol. 13:12.3.Nagy, N.et al.(2016) Avian dendritic cells: Phenotype and ontogeny in lymphoid organs.Dev Comp Immunol. 58: 47-59. |
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