| 产品介绍 | | |
| Mouse anti Human CD44v6, clone VFF-18recognizes a centrally located 14mer epitope encoded by amino acid residues 18-31, within variant exon 6 (v6) of human CD44 (Reinischet al, 1998). CD44 is a member of a polymorphic family of transmembrane glycoproteins, which exists as a broadly expressed ~80-90 kDa standard form (CD44s), and also as numerous splice variants, due to extensive alternative splicing and post-translational modifications.CD44s is involved in cell adhesion, lymphocyte activation, signal transduction, haematopoiesis, recirculation and homing, and CD44v6 mediates leukocyte to endothelial cell attachment and rolling, as well as homing to sites of inflammation and peripheral organs.As well as its role as a receptor for hyaluronic acid (HA), CD44s also interacts with other ligands including matrix metalloproteinases (MMPs), fibronectin and collagens, and has been linked with cancer of the breast, colon, stomach and squamous cell carcinomas (HNSCC) of the head and neck, as well as being a prognostic marker for non-Hodgkin's lymphoma.The variant isoforms of CD44 (CD44v) are highly expressed on epithelial cells but not leukocytes, and their association with cell transformation and cancer is also well established, supported by numerous immunohistochemical investigations including those looking at HNSCC. Mouse anti Human CD44v6, clone VFF-18 binds to CD44v6 with high affinity when compared with anti-CD44v6 clone 2F10, and is suitable for detecting CD44v6 in inflammatory bowel disease (IBD) (Reinischet al.1998). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Glutathione S Transferase (GST) fusion protein corresponding to the variable domain v6 of human CD44. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Reinisch, W.et al.(1998) Poor diagnostic value of colonic CD44v6 expression and serum concentrations of its soluble form in the differentiation of ulcerative colitis from Crohn's disease.Gut. 43 (3): 375-82.2.Foekens, J.A.et al.(1999) Prognostic value of CD44 variant expression in primary breast cancer.Int J Cancer. 84 (3): 209-15.3.Adolf, G.R. and Patzelt, E. (1999) Monoclonal antibody against CD44v6US patent pub No: US087503594.Odenthal, J.et al.(2018) Targeting CD44v6 for fluorescence-guided surgery in head and neck squamous cell carcinoma.Sci Rep. 8 (1): 10467. |
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