| 产品介绍 | | |
| Mouse anti Sheep IgE, clone 1E7, recognizes ovine Immunoglobulin E (IgE) and does not cross react with ovine IgM, IgA, IgG1 or IgG2.IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80 kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooymanet al.1997).IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Mouse anti Sheep IgE, clone 1E7 has been used to investigate parasite nematode infections of sheep byHaemonchus contortus(Verveldeet al.1997andKooymanet al.1997). Clone 1E7 has also been reported to recognize bovine IgE, and, as such, it is useful to detect response to binfection in cattle by nematode worms such asDictyocaulus viviparus(Bricarelloet al.2007andKooymanet al.2002).In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad. |
| 产品详情 | | |
| Target Species | Sheep |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Recombinant ovine IgE1-2 expressed inE.coli |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/500 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Bricarello, P.A.et al.(2007) Field study on nematode resistance in Nelore-breed cattle.Vet Parasitol.148:272-82.Vervelde, L.et al.(2003) Vaccination-induced protection of lambs against the parasitic nematodeHaemonchus contortuscorrelates with high IgG antibody responses to the LDNF glycan antigen.Glycobiology 13:795-8043.Kooyman, F.N.et al.(2002) Serum immunoglobulin E response in calves infected with the lungwormDictyocaulus viviparusand its correlation with protection.Parasite Immunol. 24:47-564.Kooyman, F.N.et al.(1997) Production of a monoclonal antibody specifc for ovine immunoglobulin E and its application to monitor serum IgE responses toHaemonchus contortusinfection.Parasitology 114:395-406 |
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