Mouse anti Rabbit GAPDH:DyLight®680
货号：MCA4739D680
规格：0.1 mg
价格：3213
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：GAPDH
物种：兔
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：Alexa Fluor 488
| 产品介绍 | | |
| Mouse anti Rabbit GAPDH antibody, clone 6C5recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Haraet al.2006). |
| 产品详情 | | |
| Target Species | Rabbit |
| Product Form | Purified IgG conjugated to DyLight®680 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from ascites |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Rabbit muscle GAPDH. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Dylight®680 | 692 | 712 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Latasa, M.U.et al.(2010) Oral methylthioadenosine administration attenuates fibrosis and chronic liver disease progression in Mdr2-/- mice.PLoS One. 5: e15690.2.Haller, S.et al.(2012) Expression profiles of metabolic enzymes and drug transporters in the liver and along the intestine of beagle dogs.Drug Metab Dispos. 40 (8): 1603-10.3.Zizza, P.et al.(2012) Phospholipase A2IVα regulates phagocytosis independent of its enzymatic activity.J Biol Chem. 287: 16849-59.4.Zschemisch, N.H.et al.(2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.BMC Immunol. 13: 60. |
技术参数 产品应用 Flow
产品类型 单克隆抗体
推荐用于 Flow
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-31257.htm
QQ:1749072012