Mouse anti Rat MHC Class II RT1Bu/L
货号：MCA45R,MCA45GA
规格：0.25 mg,0.1 mg
价格：3018,2125
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：MHC Class II RT1Bu/L
物种：大鼠
宿主：小鼠
抗体亚型：IgG1
克隆号：OX-3
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.This product is routinely tested in flow cytometry on Lewis rat splenocytes. |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG - liquid |
| Preparation | |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | Pack Size: 0.1 mg Pack Size: 0.25 mg 0.09% Sodium Azide (NaN3) |
| Immunogen | Rat thymocyte membrane glycoproteins. |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | F | 0.5mg |
| 文献 |
| 1.McMaster, W.R. & Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen.Eur J Immunol. 9 (6): 426-33.2.McMaster, W.R. & Williams, A.F. (1979) Monoclonal antibodies to Ia antigens from rat thymus: cross reactions with mouse and human and use in purification of rat Ia glycoproteins.Immunol Rev. 47: 117-37.3.Barclay, A.N. & Mayrhofer, G. (1981) Bone marrow origin of Ia-positive cells in the medulla rat thymus.J Exp Med. 153 (6): 1666-71.4.Zhang, J.et al.(1997) Expression of major histocompatibility complex molecules in rodent retina. Immunohistochemical study.Invest Ophthalmol Vis Sci. 38 (9): 1848-57.5.ahm, K.B.et al.(2000) Loss of TGF-beta signaling contributes to autoimmune pancreatitis.J Clin Invest. 105 (8): 1057-65. |
技术参数 产品应用 IHC(F);IHC(P);Flow
产品类型 Monoclonal Antibody
推荐用于 IHC(F);IHC(P);Flow
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