| 产品介绍 | | |
| Mouse anti Human F-Actin antibody, clone NH3recognizes human Filamentous actin (F-actin), the polymeric form of actin.. Mouse anti Human F-Actin antibody, clone NH3 binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgM - liquid |
| Preparation | Purified IgM prepared from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Immunogen | Human monocytes and U937 cell line. |
| Approx. Protein Concentrations | IgM concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Mouse IgM Negative Control | MCA692 | F | 100Tests |
| 文献 |
| 1.Dransfield, I.et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3).Biochem. Soc. Trans. 16: 163-164.2.Allen, K.M. & Haworth, S.G. (1989) Cytoskeletal features of immature pulmonary vascular smooth muscle cells: the influence of pulmonary hypertension on normal development.J Pathol. 158 (4): 311-7.3.McCarthy, A.M.et al.(2006) Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport.Am J Physiol Cell Physiol. 291: C860-8.4.Bhonagiri, P.et al.(2011) Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance.Endocrinology. 152: 3373-84. |
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