Rat anti mouse IgG2a HEAVY chain
货号：MCA421
规格：0.25 mg
价格：3018
产品类型：二抗
品牌：Bio-Rad
抗原：IgG2a
物种：小鼠
宿主：大鼠
抗体亚型：IgG1
克隆号：LO-MG2a-7
荧光染料：Purified
| 产品介绍 | | |
| Rat anti Mouse IgG2a:Biotin, clone LO-MG2a-7recognizes the gamma 2a heavy chain of mouse immunoglobulin and does not cross-react with other murine immunoglobulin classes or subclasses. Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-7 recognizes an allotypic determinant upon mouse IgG2a. It recognizes the IgHIa allotype (as expressed in Balb/c mice), but not the IgHIb allotype (as expressed in C57/BL mice). |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01%Thiomersal |
| Immunogen | Purified IgG from BALB/c mice. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Fusion Partners | Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5ug/ml | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Jarman, E.R. & Lamb, J.R. (2004) Reversal of established CD4+ type 2 T helper-mediated allergic airway inflammation and eosinophilia by therapeutic treatment with DNA vaccines limits progression towards chronic inflammation and remodelling.Immunology. 112 (4): 631-42.2.Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.Prot Biol Fluids 615 - 8. Pergamon Press, Oxford and New York.3.Nagao, K.et al.(2003) Role of prostaglandin I2 in airway remodeling induced by repeated allergen challenge in mice.Am J Respir Cell Mol Biol. 29: 314-20.4.Bazin, H.et al.(1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.J Immunol Methods. 66 (2): 261-9.5.Bazin, H.et al.(1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.J Immunol Methods. 71 (1): 9-16. |
技术参数 产品应用 ELISA
产品类型 Monoclonal Antibody
推荐用于 ELISA
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