Hamster anti Mouse CD27:RPE
货号：MCA4701PE
规格：0.1 mg
价格：3468
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：CD27
物种：小鼠
宿主：其它
抗体亚型：IgG1, kappa
荧光染料：PE
| 产品介绍 | | |
| Hamster anti Mouse CD27 antibody, clone LG.3A10recognizes mouse, rat and human CD27, a member of the tumor necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells, and acts as a co-stimulatory signal in lymphocyte proliferation and immunoglobulin production.CD27 lacks a death domain in its short cytoplasmic tail, but can induce apoptosis by binding with the apoptosis regulatory protein Siva (also known as CD27-binding protein). |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA. |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Approx. Protein Concentrations | IgG concentration 0.2 mg/ml |
| Fusion Partners | Armenian hamster spleen cells from immunized animals were fused with cells of the murine SP2/0 non-secreting myeloma. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control:RPE | MCA2356PE | F | 100 Tests/1ml |
| 文献 |
| 1.Gravestein, L.A.et al.(1995) Novel mAbs reveal potent co-stimulatory activity of murine CD27.Int Immunol. 7: 551-7. |
技术参数 产品应用 Flow
产品类型 单克隆抗体
推荐用于 Flow
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