| 产品介绍 | | |
| Mouse anti V5-Tag antibody, clone SV5-Pk2recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk2 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys proile pro asn pro leu leu gly leu aspser thr. (The 9 amino acid epitope is underlined). |
| 产品详情 | | |
| Target Species | Viral |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope. |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Acknowledgements | This product is manufactued under an exclusive license from the University of St. Andrews, UK. |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/100 | 1/500 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Dunn, C.et al.(1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.J Immunol Methods. 224 (1-2): 141-50.2.Boone, M.et al.(2021) Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit.Nat Commun. 12 (1): 6414. |
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