| 产品介绍 | | |
| Mouse anti Rat CD11b, clone OX-42recognizes rat CD11b, also known asintegrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a singlevWFAdomain and multipleFG-GAPrepeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes andmicrogliain the brain (Robinsonet al.1986).Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinsonet al.1986) as well as inhibit myelin binding and uptake (van der Laanet al.1996). |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to Alexa Fluor 700 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1%Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®700 | 702 | 723 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 同型对照 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control:Alexa Fluor® 700 | MCA1210A700 | F | 100 Tests/1ml |
| 文献 |
| 1.Helming, L.et al.(2009) The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion.J Cell Sci. 122 (Pt 4): 453-9.2.Mwaikambo, B.R.et al.(2009) Hypoxia up-regulates CD36 expression and function via hypoxia-inducible factor-1- and phosphatidylinositol 3-kinase-dependent mechanisms.J Biol Chem. 284: 26695-707.3.Yang, C.N.et al.(2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.Neurobiol Dis. 42 (3): 221-30.4.Seeds, R.E.et al.(2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.Int Immunopharmacol. 11: 794-801.5.Samovski, D.et al.(2012) Insulin and AMPK regulate FA translocase/CD36 plasma membrane recruitment in cardiomyocytes via Rab GAP AS160 and Rab8a Rab GTPase.J Lipid Res. 53 (4): 709-17. |
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