| 产品介绍 | | |
| Mouse anti human C1q antibody, clone 004-43.X (3R9/2),recognizes human complement component 1 q (C1q), a ~156 kDa secreted protein.C1q associates with proenzymes C1r and C1s to form the calcium-dependent C1 complex, the first component of the serum complement system. C1q is composed of six A-, six B-chains and six C-polypeptide chains. Each chain contains a collagen-like region located near the N-terminus and a C-terminal globular region. These regions bind the Fc region of IgM and IgG molecules, initiating the classical pathway of complement activation.C1q deficiency has been associated with lupus erythematosus and glomerulonephritis (Troedsonet al.2013). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from ascites |
| Buffer Solution | Borate buffered saline |
| Immunogen | Globular head domain of C1q, purified from human plasma. |
| Approx. Protein Concentrations | Current, batch-specific concentration 1.09 mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests/1ml |
| 文献 |
| 1.Petry, F. (1998) Molecular basis of hereditary C1q deficiency.Immunobiology. 199 (2): 286-94.2.Gershov, D.et al.(2000) C-Reactive protein binds to apoptotic cells, protects the cells from assembly of the terminal complement components, and sustains an antiinflammatory innate immune response: implications for systemic autoimmunity.J Exp Med. 192 (9): 1353-64.3.Castellano, G.et al.(2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage.Am J Pathol. 176: 1648-59.4.Ma, W.et al.(2012) RAGE binds C1q and enhances C1q-mediated phagocytosis.Cell Immunol. 274: 72-82.5.Lachmann, N.et al.(2013) Systematic comparison of four cell- and Luminex-based methods for assessment of complement-activating HLA antibodies.Transplantation. 95 (5): 694-700. |
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