| 产品介绍 | | |
| Mouse anti Pig CD117, clone 2B8/BMis specific for porcine CD117, also known as c-kit, a 155 kDa type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early hematopoiesis (Perezet al.2007).Hematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow hematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD).Mouse anti pig CD117, clone 2B8/BM recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells. |
| 产品详情 | | |
| Target Species | Pig |
| Product Form | Purified IgG conjugated to Alexa Fluor® 488 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Fusion Partners | Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 myeloma cell line. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Porcine bone marrow cells (BMC). |
| Approx. Protein Concentrations | IgG concentration 0.05mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Wehman, B.et al.(2017) Cardiac Progenitor Cells Enhance Neonatal Right Ventricular Function After Pulmonary Artery Banding.Ann Thorac Surg. 104 (6): 2045-53.2.Pérez Cet al.(2007) Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM.J Immunol Methods. 321 (1-2): 70-9.3.Hatzistergos, K.E.et al.(2010) Bone marrow mesenchymal stem cells stimulate cardiac stem cell proliferation and differentiation.Circ Res. 107: 913-22.4.Escalona, Z.et al.(2014) Molecular characterization and expression of porcine Siglec-5.Dev Comp Immunol. 44 (1): 206-16. |
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