Rat anti mouse Ly-6C:Biotin
货号：MCA2389B
规格：0.1 mg
价格：2550
产品类型：流式抗体
品牌：Bio-Rad
抗原：Ly-6C
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a
克隆号：ER-MP20
荧光染料：Biotin
| 产品介绍 | | |
| Rat anti Mouse CD31 antibody, clone ER-MP12recognizes mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijnet al.1998). |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Immunogen | BALB/c macrophage precursor cell hybrids |
| Buffer Solution | Phosphate buffered saline |
| Carrier Free | Yes |
| Fusion Partners | Spleen cells from immunised rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide1%Bovine Serum Albumin |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Zhang, Y. & Bliska, J.B. (2010) YopJ-promoted cytotoxicity and systemic colonization are associated with high levels of murine interleukin-18, gamma interferon, and neutrophils in a live vaccine model ofYersinia pseudotuberculosisinfection.Infect Immun 78: 2329-41.2.Leenen, P.J.et al.(1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.Eur J Immunol. 20 (1): 27-34.3.de Bruijn, M.F.et al.(1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.J Immunol Methods. 217 (1-2): 27-39.4.Schatteman, G.C.et al.(2010) Lin- Cells Mediate Tissue Repair by Regulating MCP-1/CCL-2.Am J Pathol. 177: 2002-10. |
技术参数 产品应用 Flow
产品类型 单克隆抗体
推荐用于 Flow
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