| 产品介绍 | | |
| Mouse anti Horse CD8 (clone CVS8),is a monoclonal antibody recognising the equine homologue of the human CD8 cell surface antigen which is expressed by a subset of T lymphocytes.A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim 2007). |
| 产品详情 | | |
| Target Species | Horse |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse anti Horse CD4:FITC | MCA1078F | F | 0.1 mg |
| 文献 |
| 1.Cequier, A.et al.(2022) Equine Mesenchymal Stem Cells Influence the Proliferative Response of Lymphocytes: Effect of Inflammation, Differentiation and MHC-Compatibility.Animals (Basel). 12 (8): 984.2.Lunn, D.P et al (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsetsImmunology 74: 251-257.3.Jacks, S.<i.et al.<="" i="" style="box-sizing: border-box;">(2007) Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction.Clin Vaccine Immunol. 14: 669-77.4.Pearson, W.et al.(2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.Can J Vet Res. 71: 213-7. |
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