| 产品介绍 | | |
| Mouse anti Human CD74 antibody, clone Bu45recognizes human CD74, a type II transmembrane protein, also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kDa isoforms of CD74. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes.CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. Mouse anti Human CD74 antibody, clone Bu45 binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Concentrated Tissue Culture Supernatant - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Fusion Partners | Spleen cells from immunised mice were fused with cells of the Ag8.653 myeloma cell line. |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | B lymphoblastoid cell line HFB1. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Preservative Stabilisers | 0.01%Sodium Azide |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Wraight, C.J.et al.(1990) Human major histocompatibility complex class II invariant chain is expressed on the cell surface.J Biol Chem. 265 (10): 5787-92.2.Beswick, E.J.et al.(2005)Helicobacter pyloribinds to CD74 on gastric epithelial cells and stimulates interleukin-8 production.Infect Immun. 73: 2736-43.3.Lapaque, N.et al.(2009) The HLA-DRalpha chain is modified by polyubiquitination.J Biol Chem. 284: 7007-16.4.van Bergen, J.et al.(1997) Efficient loading of HLA-DR with a T helper epitope by genetic exchange of CLIP.Proc Natl Acad Sci U S A. 94: 7499-502. |
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