| 产品介绍 | | |
| Mouse anti Bovine CD335 antibody, clone AKS1recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is expressed by human natural killer cells (Sivoriet al.1997). The bovine homologue is expressed on bovine NK cells (Storsetet al.2004) and no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells (Storsetet al.2004). |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Immunogen | Fusion protein consisting of the extracellular region of bovine CD335. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | None present |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃ only.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/5 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Storset, A.K.et al.(2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics.Eur J Immunol. 34 (3): 669-76.2.Kulberg, S.et al.(2004) Reference values for relative numbers of natural killer cells in cattle blood.Dev Comp Immunol. 28 (9): 941-8.3.Van Rhijn, I.et al.(2007) Massive, sustained γ/δ T cell migration from the bovine skinin vivo.J Leukoc Biol. 81: 968-73.4.Toka, F.N.et al.(2011) Rapid and Transient Activation of γ/δ T Cells to IFN-γ Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection.J Immunol. 186: 4853-61. |
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