| 产品介绍 | | |
| Mouse anti Human CD80 antibody, clone MEM-233recognizes human CD80, also known as B7-1, a ~60 kDa type 1 trans-membrane protein expressed of macrophages, dendritic cells (Munroet al.1994) and activated B-cells (Ranheimet al.1993). CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a singleIg-v-likedomain, a singleIg-c-likedomain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33 kDa, the presence of multiple (8) potential N-glycosylation sites (Chenet al.1998) results in a migration corresponding to ~60 kDa.Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 (Azumaet al.1993) involved in T cell activation and proliferation (Vasuet al.2003). Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics (van der Merweet al.1997).Mutagenesis indicates residues in both the Ig-v-like and Ig-c-like domains of CD80 are crucial for the interaction with it’s receptors CTLA-4 and CD28 (Peachet al.1995).Mouse anti human CD80 antibody, clone MEM-233 binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching assays (Vasuet al.2003).Mouse anti Human CD80, clone MEM-233 in combination with Mouse anti Human CD86, clone Bu63 (MCA1118) suggest that clone MEM-233 is able to block binding of human CD80 with it's cognate ligands CD28 and CTLA-4 (Morbachet al.2011). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Alexa Fluor 647 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml |
| 文献 |
| 1.Zhan, H.et al.(2003) The immunomodulatory role of human conjunctival epithelial cells.Invest Ophthalmol Vis Sci. 44 (9): 3906-10.2.Angel, C.E.et al.(2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.J Immunol. 176 (10): 5730-4.3.Daubenberger, C.A.et al.(2007) Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes ofAotus nancymaae, a non-human primate model for biomedical research.Vet Immunol Immunopathol. 119 (1-2): 14-20. |
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