Mouse anti Human CD284 antibody, clone HTA125
货号：MCA2061EL
规格：0.5 mg
价格：5100
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD284
物种：人
宿主：小鼠
抗体亚型：IgG2a
克隆号：HTA125
荧光染料：Low Endotoxin
| 产品介绍 | | |
| Mouse anti Human CD284 antibody, clone HTA125recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL) is recommended for functional assays. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Immunogen | Ba/F3 cell line expressing TLR4 (CD284). |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃ only.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Foster, N.et al.(2007) Pivotal advance: vasoactive intestinal peptide inhibits up-regulation of human monocyte TLR2 and TLR4 by LPS and differentiation of monocytes to macrophages.J Leukoc Biol. 81: 893-903.2.Shimazu, R.et al.(1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4.J Exp Med. 189 (11): 1777-82.3.Jiang, Q.et al.(2000) Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B.J Immunol. 165 (7): 3541-4.4.Yang, S.et al.(2001) Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture.Infect Immun. 69 (4): 2045-53. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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