Mouse anti Bovine CD63 antibody, clone CC25
货号：MCA2042GA
规格：0.1 mg
价格：2185
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD63
物种：Bovine
宿主：小鼠
抗体亚型：IgG1
克隆号：CC25
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Bovine CD63 antibody, clone CC25recognizes the bovine homologue of human CD63, a 237 amino acid multipass transmembrane glycoprotein and member of the tetraspanin TM4SF protein family with a predicted molecular weight of ~26 kDa. CD63 is also known as lysosome associated membrane glycoprotein 3 or LAMP-3.CD63 along with other TM4SF members including CD9, CD61 and CD151 can form specific interactions with phosphoinositide 4-kinase, suggesting a role for CD63 in the recruitment of phosphoinositide 4-kinase to specific membrane sites (Yauch and Hemler 2000). CD63 is expressed on the cell surface of platelets and basophils, along with activated macrophages, monocytes and granulocytes.Mouse anti Bovine CD63, clone CC25 acts as a specific marker for bovine lysozomes and has been used for the identification and quantitation of phagosome-lysozome fusion in models of bacterial insult (Souzaet al.2013). |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/100 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | F | 0.5mg |
| 文献 |
| 1.Brooke, G.P.et al.(1999) Molecular cloning of cattle CD63 and evidence for high level expression on subpopulations of dendritic cells.Immunogenetics. 49 (9): 812-4.2.Colino, J. and Snapper, C.M. (2006) Exosomes from bone marrow dendritic cells pulsed with diphtheria toxoid preferentially induce type 1 antigen-specific IgG responses in naive recipients in the absence of free antigen.J Immunol. 177: 3757-62.3.Souza, C.D.et al.(2007) Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes toMycobacterium aviumsubsp. paratuberculosis andMycobacterium aviumsubsp. avium.Microbes Infect. 9: 1545-52. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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