Mouse anti Bovine CD172a antibody, clone CC149
货号：MCA2041C
规格：100 Tests/1ml
价格：4208
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD172a
物种：Bovine
宿主：小鼠
抗体亚型：IgG2b
克隆号：CC149
荧光染料：PE-Cy5
| 产品介绍 | | |
| Mouse anti Bovine CD172a antibody, clone CC149recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA.CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47. Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin. |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) -Cy5 - lyophilized |
| Reconstitution | Reconstitute with 1.0ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Bovine afferent veiled dendritic cells |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE-Cy5 488nm laser | 596 | 667 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml |
| 文献 |
| 1.Howard, C.J.et al.(1999) Dendritic cells in cattle: phenotype and function.Vet Immunol Immunopathol. 72 (1-2): 119-24.2.Price, S.J. & Hope, J.C.. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture withMycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.Immunology. 126:201-83.Waters, W.R. (2009) Signal regulatory protein alpha (SIRPalpha) cells in the adaptive response to ESAT-6/CFP-10 protein of tuberculous mycobacteria.PLoS One. 4: e6414.4.Brackenbury, L.S.et al.(2005) Identification of a cell population that produces alpha/beta interferonin vitroandin vivoin response to noncytopathic bovine viral diarrhea virus.J Virol. 79: 7738-44. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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