Rat anti Mouse IgM Heavy Chain:FITC
货号：MCA199F
规格：0.5 mg
价格：5270
产品类型：荧光二抗
品牌：Bio-Rad
抗原：IgM Heavy Chain
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a
克隆号：LO-MM-9
荧光染料：FITC
| 产品介绍 | | |
| Rat anti Mouse IgM, clone LO-MM-9is directed against the mu chain of mouse IgM. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Polyclonal and monoclonal mouse IgM. |
| Fusion Partners | Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Mouse Seroblock FcR | BUF041A | F | 0.5mg |
| 文献 |
| 1.Achtman AHet al.(2003)Plasmodium chabaudi chabaudiinfection in mice induces strong B cell responses and striking but temporary changes in splenic cell distribution.J Immunol. 171 (1): 317-24.2.Bazin, H.et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.J Immunol Methods. 71: 9-16.3.Bazin, H.et al.(1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.Eur J Immunol. 4 (1): 44-8. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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