Mouse anti Dog CD4 antibody (CA13.1E4)
货号：MCA1998S
规格：2 ml
价格：3383
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD4
物种：dog
宿主：小鼠
抗体亚型：IgG1
克隆号：CA13.1E4
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) [Cobboldet al.1992] and identifies, by immunoprecipitation a ~60 kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper cells described in other mammalian species. Mouse anti Dog CD4 also recognizes a population of CD8 positive cells in the canine thymus (Mooreet al.1992).CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8 (Bismarcket al.2012). Similar reports have been made for cynomolgus monkey (Namet al.2000), pig (Saalmulleret al.1987,Pescowitzet al.1990) as well as rat, chicken and human (reviewedZuckermann 1999).Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species (Mooreet al.1992).Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone macrophages (Mooreet al.1992). Other macrophage populations, such as splenic red pulp macrophages and Langerhans cells do not stain with clone CA13.1E4. Canine CD4 positive T cells can be further characterized according to their simultaneous expression of CD45RA as recognized by Rat anti Canine anti CD45RA cloneCA4.1D3in a manner analogous to that seen with human T cells (Mooreet al.1992).Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for the study of various lymphoproliferative diseases. T lymphocytes in large granular lymphocyte (LGL) lymphocytosis are negative for CD4 (McDonough and Moore 2000). Dogs with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils (Tarrantet al.2001). CD4 expression varies considerably between various canine leukemias. |
| 产品详情 | | |
| Target Species | Dog |
| Product Form | Tissue Culture Supernatant - liquid |
| Preservative Stabilisers | 0.1%Sodium Azide |
| Immunogen | Canine Thymocytes |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 mouse myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Moore, P.F.et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils.Tissue Antigens 40(2): 75-85.2.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.3.Veenhof, E.Z.et al.(2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.Vet J. 187 (3): 320-4.4.Izci Cet al.(2015) Clinical and light microscopic studies of the conjunctival tissues of dogs with bilateral keratoconjunctivitis sicca before and after treatment with topical 2% cyclosporine.Biotech Histochem. 90 (3): 223-30. |
技术参数 产品应用 Flow；IHC
产品类型 Monoclonal Antibody
推荐用于 Flow；IHC
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