| 产品介绍 | | |
| Mouse anti Human estrogen receptor beta 1, clone PPG5/10recognizes the estrogen receptor beta 1 (ERB1), one of several isoforms of estrogen receptor (ER) which can be used in conjunction with progesterone receptor (PR) and HER2/neu (CD340), to help determine both prognosis and the effectiveness of hormonal therapy, in breast cancer patients. Prognosis and survival rate varies greatly depending on cancer type, stage and treatment.Mouse anti Human estrogen receptor beta 1, clone PPG5/10 is increasingly being utilized in the immunohistochemical staining of formal fixed paraffin embedded tissue, in preference to ligand binding assays, for the detection of ER/PR. ER/PR is present in approximately 75% to 80% of breast tumors, and breast cancer cells with these receptors depend on the hormones estrogen and/or progesterone to grow (Saunderset al., 2002andNelsonet al., 2017).Mouse anti Human estrogen receptor beta 1, clone PPG5/10 can be used to demonstrate the nuclear presence of ERB1 within cells of the breast (Shaabanet al., 2008), ovary, uterus, oviduct, testis, prostate, bladder and male reproductive ducts. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Synthetic peptide sequence CSPAEDSKSKEGSQNPQSQ from the C-terminal region of the human estrogen receptor beta 1 isoform. |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunofluorescence | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Miller, W.R.et al.(2006) Oestrogen receptor beta and neoadjuvant therapy with tamoxifen: prediction of response and effects of treatment.Br J Cancer. 94: 1333-8.2.Nakopoulou, L.et al.(2004) The favourable prognostic value of oestrogen receptor beta immunohistochemical expression in breast cancer.J Clin Pathol. 57: 523-8.3.Rago, V.et al.(2007) Cytochrome P450arom, androgen and estrogen receptors in pig sperm.Reprod Biol Endocrinol. 5: 23. |
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