| 产品介绍 | | |
| Rat anti Mouse M31 antibody, clone MAC353recognises the M31 molecule in mouse and the homologous Chromobox protein homolog 1 or HP1Hsbeta molecule in man. Rat anti Mouse M31 antibody, clone MAC353 has been reported for use in western blotting and recognises a ~26 kDa protein in murine and human nuclear extracts (Wreggettet al.1994). |
| 产品详情 | | |
Target Species | Mouse |
| Species Cross-Reactivit | Target Species | Cross Reactivity |
| Human | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Tissue Culture Supernatant - liquid |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3) |
Immunogen | 71 amino acid fusion protein corresponding to the C-terminal region of M31 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom dateof despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
| Immunofluorescence | √ | | |
| Chromatin Immunoprecipitation | √ | | |
| Immunohistology - Frozen | √ | Neat | 1/50 |
| Immunoprecipitation | √ | | |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Wreggett, K.A.et al.(1994) A mammalian homologue ofDrosophilaheterochromatin protein 1 (HP1) is a component of constitutive heterochromatin.Cytogenet Cell Genet. 66 (2): 99-103.2. Sabbattini, P.et al.(2007) A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.EMBO J. 26 (22): 4657-69.3. Epsztejn-Litman, S.et al.(2008)De novoDNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes.Nat Struct Mol Biol. 15: 1176-83.4. Matsui, T.et al.(2010) Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET.Nature. 464: 927-31.5. Frohnert, C.et al.(2011) SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome.Mol Hum Reprod. 17: 207-18.6. Baxter, J.et al.(2004) Histone hypomethylation is an indicator of epigenetic plasticity in quiescent lymphocytes.EMBO J. 23 (22): 4462-72.7. McDowell, T.L.et al.(1999) Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.Proc Natl Acad Sci U S A. 96: 13983-8.8. Sabbattini, P.et al.(2007) A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.EMBO J. 26 (22): 4657-69.9. Groves, I.J.et al.(2009) Lytic infection of permissive cells with human cytomegalovirus is regulated by an intrinsic 'pre-immediate-early' repression of viral gene expression mediated by histone post-translational modification.J Gen Virol. 90: 2364-74.10. Groves, I.J. and Sinclair, J.H. (2007) Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression.J Gen Virol. 88: 2935-40.11. Abe, K.et al.(2011) Loss of heterochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell cycle progression in mice.Biol Reprod. 85 (5): 1013-24. |
QQ:1749072012