| 产品介绍 | | |
| Mouse anti Human IgM antibody, clone CM7is an excellent broad species anti-IgM monoclonal antibody, and shows no cross-reactivity with other immunoglobulin classes or sub classes.Mouse anti Human IgM antibody, clone CM7 may be used as a marker of mature B lymphocytes in flow cytometry. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Horse | √ |
| Pig | √ |
| Marmoset | √ |
| Dog | √ |
| Cat | √ |
| Rhesus Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide |
| Immunogen | Purified human IgM, and then purified cat IgM. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| Flow Cytometry | √ | 1/10 | 1/50 |
| Immunohistology - Paraffin | √ | | |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood.
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| 文献 |
| 1. Reubel, G.H.et al.(1992) Interaction of acute feline herpesvirus-1 and chronic feline immunodeficiency virus infections in experimentally infected specific pathogen free cats.Vet Immunol Immunopathol. 35 (1-2): 95-119.2. Lunn, D.P.et al.(1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.Vet Immunol Immunopathol. 62 (2): 101-43.3. Chandhasin, C.et al.(2005) Unique long terminal repeat and surface glycoprotein gene sequences of feline leukemia virus as determinants of disease outcome.J Virol. 79 (9): 5278-87.4. Prieto, J.M.et al.(2017) Developmental expression of B cell molecules in equine lymphoid tissues.Vet Immunol Immunopathol. 183: 60-71.5. Tallmadge, R.L.et al.(2012) Expression of essential B cell development genes in horses with common variable immunodeficiencyMolecular Immunology. 51 (2): 169-76. |
QQ:1749072012