| 产品介绍 | | |
| Rat anti Mouse CD252 antibody, clone OX-89recognizes mouse OX40 (CD134) ligand, which is otherwise known as CD252. CD252 expression has been shown to be restricted to activated dendritic cells, B cells and vascular endothelium.Rat anti Mouse CD252 antibody, clone OX-89 has been reported to inhibit OX40/OX40L interaction (Malströmet al.2001). |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | Purified IgG- liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
| Immunogen | Rat CD4 and mouse CD134 ligand Chimaeric protein. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Fusion Partners | Spleen cells from immunized PVG rats were fuzed with cells of the NS1 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| Immunohistology - Frozen | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| 数据 |
| Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD252 antibody, clone OX-89 (MCA1881) followed by Goat anti Rat IgG antibody (STAR72). High power |
| 文献 |
| 1. Malmström Vet al.(2001) CD134L expression on dendritic cells in the mesenteric lymph nodes drives colitis in T cell-restored SCID mice.J Immunol. 166 (11): 6972-81. |
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