| 产品介绍 | | |
| Mouse anti Human CD147 antibody, clone MEM-M6/1recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal)Ig-like C2-type domainamd a more proximalIg-like V-type domainin its extracellular region. Mouse anti Human CD147 antibody, clone MEM-M6/1 was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells, along with a number of other MEM-M6 clones. Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domain. This is confirmed by flow cytometry using beads coated with constructs containing only the distal Ig-like or proximal Ig-like domains (Kochet al.1999).Mouse anti Human CD147 antibody, clone MEM-M6/1 binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin (Kochet al.1999). In addition to clone MEM-M6/1 we are also able to offer theMEM-M6/6 clonederived from the same fusion which recognizes an epitope in the membrane proximal Ig-like V type domain and has inhibitory effects on CD3 induced T cell activation (Kock et al. 1999) |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose |
| Immunogen | CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147. |
| Fusion Partners | Spleen cells from immunized Balb/c mice were fused with SP2/0 mouse myeloma cells. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE | MCA928PE | Flow | 100 Tests |
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| 文献 |
| 1. Koch, C.et al.(1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density.Int Immunol. 11 (5): 777-86.2. Yang, Y.et al.(2008) Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis.Rheumatology (Oxford). 47: 1299-310.3. Schneiderhan, W.et al.(2007) Pancreatic stellate cells are an important source of MMP-2 in human pancreatic cancer and accelerate tumor progression in a murine xenograft model and CAM assay.J Cell Sci. 120: 512-9.4. Chen, Y.et al.(2009) Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis.Cancer Lett. 278: 113-21.5. Schneiderhan, W.et al.(2009) CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro modelsGut. 58: 1391-8.6. Wright, G.J.et al.(2014) Immunogenic compositions and expression systems.Patent Publication number. US20140093540 A17. Huang, Z.et al.(2013) Overexpression of CD147 contributes to the chemoresistance of head and neck squamous cell carcinoma cells.J Oral Pathol Med. 42: 541-6.8. Hu, J.et al.(2010) Involvement of HAb18G/CD147 in T cell activation and immunological synapse formation.J Cell Mol Med. 14 (8): 2132-43.9. Bernard, S.C.et al.(2014) PathogenicNeisseria meningitidisutilizes CD147 for vascular colonization.Nat Med. 20 (7): 725-31.10. Nassif, X.et al.(2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia.Patent Publication number US20150110806 A111. Trakarnsanga, K.et al.(2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.Nat Commun. 8: 14750. |
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