| 产品介绍 | | |
| Mouse anti Human MCM2 antibody, clone CRCT2.1recognizes human DNA replication licensing factor MCM2, also known as Mcm2, Nuclear protein BM28 or mini chromosome maintenance protein-2. Mcm-2 is a 904 amino acid ~125kDa nuclear protein involved in the control of DNA replication.Mouse anti Human MCM2 antibody, clone CRCT2.1 has been used successfully for the detection of human MCM2 in ovarian adenocarcinoma by immunohistochemistry on formalin fixed, paraffin embedded tissues (Gakiopoulouet al.2007). |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG- liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide |
| Carrier Free | Yes |
| Immunogen | Bacterially expressed human Mcm2. |
| Approx. Protein Concentrations | IgG concentration1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised CBA/c F1 mice were fused with cells of the mouse SP-2/0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | Guaranteed for 12 months from the date of despatch . |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunohistology - Paraffin1 | √ | | 1/200 |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Histology Positive Control TissueTonsil, any tissue with high proliferative index
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| 数据 |
| Paraffin embedded human tonsil stained with Mouse anti Human MCM2 (MCA1859) following citrate antigen retrieval. |
| 文献 |
| 1. (2011) Human Protein Atlas: CAB000303Human Protein Atlas: CAB0003032. Gakiopoulou, H.et al.(2007) Minichromosome maintenance proteins 2 and 5 in non-benign epithelial ovarian tumours: relationship with cell cycle regulators and prognostic implications.Br J Cancer. 97:1124-34.3. Korkolopoulou, P.et al.(2005) Minichromosome maintenance proteins 2 and 5 expression in muscle-invasive urothelial cancer: a multivariate survival study including proliferation markers and cell cycle regulators.Hum Pathol. 36: 899-907.4. Giaginis, C.et al.(2009) Clinical significance of MCM-2 and MCM-5 expression in colon cancer: association with clinicopathological parameters and tumor proliferative capacity.Dig Dis Sci. 54: 282-91.5. Polo, S.E.et al.(2010) Clinical significance and prognostic value of chromatin assembly factor-1 overexpression in human solid tumours.Histopathology. 57: 716-24.6. FitzGerald, J.et al.(2014) A high through-put screen for small molecules modulating MCM2 phosphorylation identifies Ryuvidine as an inducer of the DNA damage response.PLoS One. 9: e98891.7. Almouzni, G.et al.(2011) Proliferation markers in clinical practice and their use for breast cancer prognosis or diagnosis.U.S. Patent Application: US 20110003706 A18. Montes de Oca, R.et al.(2015) The histone chaperone HJURP is a new independent prognostic marker for luminal A breast carcinoma.Mol Oncol. 9 (3): 657-74.9. Wu, K.Z.et al.(2016) DDK dependent regulation of TOP2A at centromeres revealed by a chemical genetics approach.Nucleic Acids Res. 44 (18): 8786-8798. |
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