| 产品介绍 | | |
| Mouse anti Bovine GM-CSF antibody, clone CC305recognizes bovine granulocyte macrophage colony-stimulating factor (GM-CSF) also known as CSF-2. Bovine GM-CSF is a 143 amino acid cytokine which stimulates proliferation and differentiation of hematopoietic precursors.Mouse anti Bovine GM-CSF antibody, clone CC305 has been used as a capture antibody in conjunction with Mouse anti Sheep GM-CSF, clone 3C2 as a detection reagent in a sandwich ELISA for the measurement of bovine GM-CSF produced by peripheral blood mononuclear cellsin vitro(Kennedyet al.2002). |
| 产品详情 | | |
Target Species | Bovine |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Sheep | |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
| Immunogen | T-end.1, a pMT transformed endothelial cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised C57/BL mice were fused with cells of the mouse SP2/0 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 5 ug/ml as coating antibody |
| Western Blotting | | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- ELISAThis product may be used as a capture antibody in a sandwich ELISA together withMCA1924when conjugated to biotin as the detection reagent.
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| 数据 |
| Bovine GM-CSF ELISARecombinant Bovine GM-CSF detected using Mouse anti Bovine GM-CSF (MCA1828GA) as the capture reagent and a bovine reactive Mouse anti Sheep GM-CSF, clone 3C2 (MCA1924) conjugated to Biotin as the detection reagent followed by Streptavidin:HRP |
| 文献 |
| 1. Kennedy HEet al.(2002) Modulation of immune responses toMycobacterium bovisin cattle depleted of WC1(+) gamma delta T cells.Infect Immun. 70 (3): 1488-500.2. Boutet, P.et al.(2004) Delayed neutrophil apoptosis in bovine subclinical mastitis.J Dairy Sci. 87 (12): 4104-14.3. Bao, Z.J.et al.(2014) Recombinant bovine interferon-τ enhances in vitro development of bovine embryos by upregulating expression of connexin 43 and E-cadherin.J Dairy Sci. 97 (11): 6917-25.4. Pedersen, L.G.et al.(2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal speciesVet Immunol Immunopathol. 88: 111-22.5. Davies, C.J.et al.(2004) Methods of minimizing immunological rejection of a nuclear transfer fetus.U.S. Patent Application: US 2004/0029825 A1 |
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