| 产品介绍 | | |
| Mouse anti Human CD89 antibody, clone MIP8arecognizes the human CD89 cell surface antigen, a 50-75 kDa cell surface glycoprotein that is also known as the IgA receptor (Fc alpha R).CD89 is expressed by peripheral blood monocytes and neutrophils.MIP8a blocks binding of IgA to the Fc alpha R, and also inhibits neutrophil phagocytosis of IgA complexes. |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Immunogen | Recombinant soluble human Fc alpha R. |
| Fusion Partners | Lymph node cells from immunised Armenian hamsters were fused with cells of the mouse X63-Ag8.653 myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/50 | 1/100 |
| ELISA | √ | | |
| Immunoprecipitation | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood
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| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
| 文献 |
| 1. Hamre, R.et al.(2003) Expression and modulation of the human immunoglobulin A Fc receptor (CD89) and the FcR gamma chain on myeloid cells in blood and tissue.Scand J Immunol. 57: 506-16.2. Qian,K.et al.(2008) Functional expression of IgA receptor FcalphaRI on human platelets.J Leukoc Biol. 84: 1492-500.3. Van Egmond, M. (2011) Method for the treatment or prophylaxis of chronic inflammatory diseases.European Patent Application No: 12/7369634. Pascal, V.et al.(2012) Anti-CD20 IgA can protect mice against lymphoma development: evaluation of the direct impact of IgA and cytotoxic effector recruitment on CD20 target cells.Haematologica. 97 (11): 1686-94.5. Mladenov, R.et al.(2015) The Fc-alpha receptor is a new target antigen for immunotherapy of myeloid leukemia.Int J Cancer. 137 (11): 2729-38.6. Aleyd, E.et al.(2016) IgA Complexes in Plasma and Synovial Fluid of Patients with Rheumatoid Arthritis Induce Neutrophil Extracellular Traps via FcαRI.J Immunol. 197 (12): 4552-9.7. van der Steen, L.et al.(2009) Immunoglobulin A: Fc(alpha)RI interactions induce neutrophil migration through release of leukotriene B4.Gastroenterology. 137 (6): 2018-29.e1-3.8. Hamre, R.et al.(2003) Expression and modulation of the human immunoglobulin A Fc receptor (CD89) and the FcR gamma chain on myeloid cells in blood and tissue.Scand J Immunol. 57 (6): 506-16.9. Lu, J.et al.(2014) Pentraxins and IgA share a binding hot-spot on FcαRI.Protein Sci. 23 (4): 378-86.10. Askarian, F.et al.(2021) The lytic polysaccharide monooxygenase CbpD promotesPseudomonas aeruginosa.virulence in systemic infection.Nat Commun. 12 (1): 1230. |
QQ:1749072012