| 产品介绍 | | |
| Mouse anti Bovine Interleukin-4 antibody, clone CC303recognizes bovine interleukin 4 |
| 产品详情 | | |
Target Species | Bovine |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Dog | √ |
| Pig | √ |
| Sheep | √ |
| Mustelid | √ |
| Goat | √ |
| Dolphin | √ |
| Mink | √ |
| Fin Whale | √ |
| Horse | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry1 | √ | 1/50 | 1/100 |
| ELISA | √ | | |
| Western Blotting | X | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
|
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| Human Seroblock | BUF070B | F | 200 Test |
| 文献 |
| 1. Costa-Pereira, C.et al.(2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.BMC Vet Res. 11 (1): 92.2. Dean, G.S.et al.(2005) Minimum infective dose ofMycobacterium bovisin cattle.Infect Immun. 73 (10): 6467-71.3. Araújo, M.S.et al.(2009) T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune.Vaccine. 27 (7): 1008-17.4. Yang, J.et al.(2012) Comparison of worm development and host immune responses in natural hosts ofSchistosoma japonicum, yellow cattle and water buffalo.BMC Vet Res. 8: 25.5. Moreira, M.L.et al.(2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.Vet Parasitol. 220: 33-45.6. Geherin, S.A.et al.(2013) Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7.Vet Immunol Immunopathol. 155 (1-2): 87-97.7. Aguiar-Soares, R.D.O.et al.(2020) Phase I and II Clinical Trial Comparing the LBSap, Leishmune®, and Leish-Tec®Vaccines against Canine Visceral Leishmaniasis.Vaccines (Basel). 8 (4)Nov 17 [Epub ahead of print].8. Blanco, C.F.et al.(2021) Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression systemJournal of Veterinary Research. 65, Aug 19 [Epub ahead of print]9. Villa-Mancera, A.et al.(2021) Phage display-based vaccine with cathepsin L and excretory-secretory products mimotopes ofFasciola hepatica.induces protective cellular and humoral immune responses in sheep.Vet Parasitol. 289: 109340. |
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