| 产品介绍 | | |
| Mouse anti Dog IgE antibody, clone E6-71A1recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of ~62-65 kDa under reducing conditions. |
| 产品详情 | | |
| Target Species | Dog |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Affinity purified IgE preparation from heavily parasitized and allergic dog serum. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Ognjenovic, J.et al.(2013) Immunoproteomic characterization ofAmbrosia artemisiifoliapollen allergens in canine atopic dermatitis.Vet Immunol Immunopathol. 155: 38-47.2.Martins, L.M.et al.(2017) Allergy to grass pollen: mapping ofDactylis glomerataandPhleum pratenseallergens for dogs by two-dimensional immunoblotting.Postepy Dermatol Alergol. 34 (1): 60-9. |
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