| 产品介绍 | | |
| Mouse anti Dog CD11b antibody, clone CA16.3E10is a monoclonal antibody recognizing the canine CD11b cell surface antigen, a member of the alpha integrin family. CD11b forms one of the possible alpha chains of the canine leukocyte adhesion complexes (LeuCAMs), these contain a common 95 kDa β chain (CD18) non-covalently bound to either a 150 kDa (CD11c), 165 kDa (CD11b) or 180 kDa (CD11a) α chain (Mooreet al.1990. The CD11/CD18 complex is also known as the CR3 receptor.Canine CD11b is expressed by granulocytes, monocytes, NK cells and some macrophages. Mouse anti Dog CD11b antibody, clone CA16.3E10 has been used to evaluate the effect of anesthetic administration of CD11b expression on canine neutrophils (Maedaet al.2010) demonstrating attenuation of CD11b expression at high concentrations administered lidocaine hydrochloride and reduced adhesion of neutrophils to endothelium. |
| 产品详情 | | |
| Target Species | Dog |
| Product Form | Tissue Culture Supernatant - liquid |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Affinity purified beta-2 integrins from splenic lysate |
| Approx. Protein Concentrations | <0.1% Sodium Azide (NaN3) |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Danilenko, D.M.et al. (1992) Canine leukocyte cell adhesion molecules (LeuCAMs): characterization of the CD11/CD18 family.Tissue Antigens 40: 13-21.2.Brodersen, R.et al.(1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.Vet Immunol Immunopathol. 64 (1): 1-13.3.Sampaio, W.M. (2007) In vitro binding and survival assays ofLeishmaniaparasites to peripherical blood monocytes and monocyte-derived macrophages isolated from dogs naturally and experimentally infected withLeishmania chagasi.BMC Vet Res. 3:11. |
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