mouse anti human CD44v7/8:FITC
货号：MCA1732F
规格：100 Tests
价格：3247
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD44v7/8
物种：人
宿主：小鼠
抗体亚型：IgG2b, kappa
克隆号：VFF-17
荧光染料：FITC
产品介绍 | | |
| Mouse anti Human CD44v7/8 antibody, clone VFF-17specifically recognizes an epitope encoded by exons v7-v8 on the variant portion of human CD44. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Buffer Solution | Tris buffered saline |
| Preservative Stabilisers | 0.02%Sodium Azide 1%Bovine Serum Albumin |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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同型 |
Description | Product Code | Applications | Pack Size |
Mouse IgG2b Negative Control:FITC | MCA691F | Flow | 100 Tests |
| 文献 |
| 1. Wibulswas, A.et al.(2000) The CD44v7/8 epitope as a target to restrain proliferation of fibroblast-like synoviocytes in rheumatoid arthritis.Am J Pathol. 157 (6): 2037-44.2. Alam, T.N.et al.(2004) Differential expression of CD44 during human prostate epithelial cell differentiation.J Histochem Cytochem. 52: 1083-90.3. Hanley, W.D.et al.(2006) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.FASEB J. 20: 337-9.4. Rajarajan, A.et al.(2012) CD44 expression in oro-pharyngeal carcinoma tissues and cell lines.PLoS One. 7: e28776.5. Bauer, S.et al.(2006) Fibroblast activation protein is expressed by rheumatoid myofibroblast-like synoviocytes.Arthritis Res Ther. 8: R171.6. Shirure, V.S.et al.(2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.Am J Physiol Cell Physiol. 308 (1): C68-78.7. Noori, M.S.et al.(2018) An adhesion based approach for the detection of esophageal cancer.Integr Biol (Camb). 10 (12): 747-57. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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