Mouse anti Pig CD4 Alpha:RPE
货号：MCA1749PE
规格：100 Tests
价格：4522
产品类型：流式抗体
品牌：Bio-Rad
物种：pig
宿主：小鼠
抗体亚型：IgG2b
克隆号：MIL17
荧光染料：PE
| 产品介绍 | | |
| Mouse anti Porcine CD4 alpha, clone MIL17recognizes a ~55 kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haversonet al.2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily.Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmulleret al.1987)Mouse anti Pig CD4 alpha, clone MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry |
| 产品详情 | | |
| Target Species | Pig |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1.0 ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide1%Bovine Serum Albumin |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Immunogen | Leucocytes isolated from porcine gut lamina propria. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2b Negative Control:RPE | MCA691PE | F | 100 Tests |
| 文献 |
| 1.Saalmüller Aet al.(2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.Vet Immunol Immunopathol. 80 (1-2): 35-52.2.Castellano, G.et al.(2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage.Am J Pathol. 176: 1648-59.3.Inman, C.F.et al.(2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.J Leukoc Biol. 88 (3): 571-8.4.Kick ARet al.(2011) Evaluation of peripheral lymphocytes after weaning and vaccination forMycoplasma hyopneumoniae.Res Vet Sci. 91 (3): e68-72. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-32753.htm
QQ:1749072012