产品介绍 | | |
| Mouse anti Human CD44v7 antibody, clone VFF-9specifically recognizes an epitope encoded by exon v7 on the variant portion of human CD44. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44. |
| Approx. Protein Concentrations | IgG concentration 1mg/ml |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the P3X63Ag8.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | 1/50 |
| Immunohistology - Frozen | √ | | 1/50 |
| Immunohistology - Paraffin1 | √ | | 1/50 |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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同型 |
Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control | MCA928 | Flow | 100 Tests |
| 文献 |
| 1. Bànkfalvi, A.et al.(1998) Gains and losses of CD44 expression during breast carcinogenesis and tumour progression.Histopathology. 33 (2): 107-16.2. Koopman, G.et al.(1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.J Exp Med. 177 (4): 897-904.3. Kim, J.S. and Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease.J Histochem Cytochem. 59: 780-90.4. Rajarajan, A.et al.(2012) CD44 expression in oro-pharyngeal carcinoma tissues and cell lines.PLoS One. 7: e28776.5. Hanley, W.D.et al.(2005) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.FASEB J. 20: 337-9.6. Kim, J.S.et al.(2012) Gottron's papules exhibit dermal accumulation of CD44 variant 7 (CD44v7) and its binding partner osteopontin: a unique molecular signature.J Invest Dermatol. 132 (7): 1825-32.7. Shirure, V.S.et al.(2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.Am J Physiol Cell Physiol. 308 (1): C68-78.8. Noori, M.S.et al.(2018) An adhesion based approach for the detection of esophageal cancer.Integr Biol (Camb). 10 (12): 747-57. |
QQ:1749072012