Mouse anti Human CD227:FITC
货号：MCA1742F
规格：0.1 mg
价格：2865
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD227
物种：人
宿主：小鼠
抗体亚型：IgG3
克隆号：C595 (NCRC48)
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Human CD227 antibody, clone C595 (NCRC48)recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Urinary MUC-1 mucin. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1%Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Tests |
| 文献 |
| 1.Cabañas, C.et al.(1989) Characterization of a novel myeloid antigen regulated during differentiation of monocytic cells.Eur J Immunol. 19 (8): 1373-8.2.Fawcett, J.et al. (1995) Mapping the homotypic binding sites in CD31 and the role of CD31 adhesion in the formation of interendothelial cell contacts.J Cell Biol. 128:1229-41.3.Paul, G.et al.(2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.PLoS One. 7: e35577. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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