| 产品介绍 | | |
| Mouse anti Human CD227 antibody, clone C595 (NCRC48)recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti Human CD227 antibody, clone C595 recognizes the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Carrier Free | Yes |
| Immunogen | Urinary MUC-1 mucin. |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line. |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/100 | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5mg |
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E,WB | 0.5mg |
| 文献 |
| 1.Price, M.R.et al.(1990) Immunological and structural features of the protein core of human polymorphic epithelial mucin.Mol Immunol. 27 (8): 795-802.2.Price, M.R.et al.(1990) C595--a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas.Br J Cancer. 61 (5): 681-6.3.Price, M.R.et al.(1991) Purification of anti-epithelial mucin monoclonal antibodies by epitope affinity chromatography.J Immunol Methods. 139 (1): 83-90.4.Denton, G.et al.(1995) Primary sequence determination and molecular modelling of the variable region of an antiMUC1 mucin monoclonal antibody.Eur J Cancer. 31A (2): 214-21. |
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