| 产品介绍 | | |
| Mouse anti Human CD54 antibody, clone 15.2recognizes the human CD54 cell surface antigen also known as intracellular Adhesion Molecule -1 (ICAM-1) or Major group rhinovirus receptor.CD54 is expressed by many cells following activation by inflammatory mediators. It is a 505 amino acid with an additional 27 amino acid signal peptide ~90 kDa single pass type I transmembrane glycoprotein bearing 5 Ig-like C2-type domains.Mouse anti Human CD54 antibody, clone 15.2 blocks CD54 function (Berendtet al.1992). Mouse anti Human CD54 antibody, clone 15.2 binds to an epitope on the N-terminal Ig-like domain within a region designated the L43 loop (Chakravorty and Craig 2005). |
| 产品详情 | | |
| Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Pig | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
| Preparation | - MCA1615, MCA1615T: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA1615GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
|
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Human monocytes |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0-Ag14 myeloma cell line |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/50 | 1/100 |
| Immunohistology - Frozen | √ | 1/50 | 1/100 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5mg |
| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | E,WB | 0.5mg |
| 文献 |
| 1.Dransfield, I.et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.J Cell Biol. 116:1527-35.2.Berendt, A.et al. (1992) The binding site on ICAM-1 for plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1- binding site.Cell. 68: 71-81.3.Urquhart, P.et al.(2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.J Pharmacol Exp Ther. 321 (2): 656-62. |
QQ:1749072012