RevertAid™ H Minus First Strand cDNA Synthesis Kit, with DNase I/RevertAid™第一链cDNA合成试剂盒（含DNase I）

货号：K16315,K16325

规格：20 reactions,100 reactions

价格：0,1687

产品类型：核酸合成

品牌：Thermo Fisher

The Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit (with DNase I) is a complete system for efficient synthesis of first-strand cDNA from RNA templates. The kit includes recombinant endonuclease (DNase I) to remove contaminating genomic DNA from RNA preps.For reverse transcription the kit uses RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

优点：

- Integrated genomic DNA removal step- High yields of full-length first-strand cDNA up to 13 kb- Increased reaction temperatures in the range of 42 to 55°C- Supplied with the recombinant RiboLock RNase Inhibitor- Complete kit—oligo(dT)18 and random hexamer primers included with the kit

组成成分：

▪1 x 25 µL RevertAid H Minus Reverse Transcriptase (200 U/µL)▪1 x 25 µL RiboLock RNase Inhibitor (20 U/µL)▪1 x 150 µL 5X Reaction Buffer▪1 x 50 µL dNTP Mix (10 mM)▪1 x 25 µL Oligo(dT)18 Primer (100 µM)▪1 x 25 µL Random Hexamer Primer (100 µM)▪1 x 20 µL Control GAPDH RNA (0.05 µg/µL)▪1 x 20 µL Forward GAPDH Primer (10 µM)▪1 x 20 µL Reverse GAPDH Primer (10 µM)▪2 x 1.25 mL water, nuclease-free▪1 x 20 µL DNase I, RNase-free (1 U/µL)Store at -20°C.

相关产品：

▪RevertAid cDNA第一链合成试剂盒（含DNase I）▪RevertAid H Minus第一链cDNA合成试剂盒（含DNase I）▪RevertAid 预混液（含DNase I）

技术参数

产品应用 • First strand cDNA synthesis for RT-PCR and real-time RT-PCR • Construction of full length cDNA libraries • Antisense RNA synthesis

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-32920.htm